4D biofabrication via instantly generated graded hydrogel scaffolds

Biofabrication of Tissue Scaffolds

Microbubbles as biocompatible porogens for hydrogel scaffolds.

In this study, we explored the application of lipid-shelled, gas-filled microbubbles as a method for creating on-demand microporous hydrogels for cartilage tissue engineering. The technique allowed for homogenous distribution of cells and micropores within the scaffold, increasing the absorption coefficient of large solutes (70kDa dextran) over controls in a concentration-dependent manner. The ...

Computer Generated Holography with Intensity-Graded Patterns

Computer Generated Holography achieves patterned illumination at the sample plane through phase modulation of the laser beam at the objective back aperture. This is obtained by using liquid crystal-based spatial light modulators (LC-SLMs), which modulate the spatial phase of the incident laser beam. A variety of algorithms is employed to calculate the phase modulation masks addressed to the LC-...

Biofabrication with biopolymers and enzymes: potential for constructing scaffolds from soft matter.

PURPOSE Regenerative medicine will benefit from technologies capable of fabricating soft matter to have appropriate architectures and that provide the necessary physical, chemical and biological cues to recruit cells and guide their development. The goal of this report is to review an emerging set of biofabrication techniques and suggest how these techniques could be applied for the fabrication...

Fabrication of alginate hydrogel scaffolds and cell viability in calcium-crosslinked alginate hydrogel

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